|本期目录/Table of Contents|

[1]冶娟,慕晓玲△.人脐带、胎盘间充质干细胞样细胞 中“干”性蛋白表达的研究[J].生物医学工程研究,2011,04:220-225.
 YE Juan,MU Xiaoling.Expression of Stem Cell Protein in the Mesenchymal Stem Cell-like Cells Derived from Human Umbilical Cord and Placenta[J].Journal of Biomedical Engineering Research,2011,04:220-225.
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人脐带、胎盘间充质干细胞样细胞 中“干”性蛋白表达的研究(PDF)

《生物医学工程研究》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2011年04期
页码:
220-225
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Expression of Stem Cell Protein in the Mesenchymal Stem Cell-like Cells Derived from Human Umbilical Cord and Placenta
作者:
冶娟慕晓玲△
新疆石河子大学医学院/新疆地方与民族高发病实验室,新疆 石河子 832000
Author(s):
YE Juan MU Xiaoling
Xingjiang Shihezi Medical University/Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi 832000,China
关键词:
MRP1ABCG2P75NTRNestin胎盘脐带间充质干细胞
Keywords:
MRP1 ABCG2 P75NTR Nestin Placenta Umbilical cord Mesenchymal stem cell
分类号:
R318
DOI:
-
文献标识码:
A
摘要:
检测原代分离培养的人脐带、胎盘间充质干细胞 样细胞中CD29、CD34、CD44、CD133、HLA-DR、vWF、MRP1、ABCG2、P75NTR及Nestin的表达。采用组织块贴壁培养法分离培养人脐带、胎 盘间充质干细胞样细胞,通过培养扩增后用流式细胞仪检测CD29、CD34、CD44、CD133、HLA-DR及vWF的表达,应用免疫荧光技术检测人脐 带、胎盘间充质干细胞样细胞中MRP1、ABCG2、P75NTR、Nestin的表达。在培养的人脐带、胎盘间充质干细胞样细胞中,流式细胞仪检测 示CD29、CD44表达阳性,CD34、CD133、HLA-DR及vWF表达阴性;免疫荧光示MRP1、ABCG2、P75NTR及Nestin在人脐带、胎盘间充质干细胞 样细胞中表达阳性且荧光定位在细胞的胞浆。人脐带、胎盘间充质干细胞样细胞表达间充质干细胞的免疫表型;MRP1、ABCG2、P75NTR、 Nestin在人脐带、胎盘间充质干细胞样细胞中表达阳性,提示人脐带与胎盘来源的间充质干细胞样细胞“干”性蛋白的表达情况相似。
Abstract:
To detect the expression of CD29, CD34, CD44, CD133, HLA-DR, vWF, MRP1, ABCG2, P75NTR and Nestin in mesenchymal stem cell-like cells isolated from human umbilical cord and placenta, respectively.The mesenchymal stem cell-like cells were isolated from human umbilical cord and placenta by tissue adherence, respectively. The surface antigens of the cultured cells were detected by flow cytometry and immunofluorescence in mesenchymal stem cell-like cells derived from human umbilical cord and placenta, respectively.The mesenchymal stem cell-like cells derived from umbilical cord and placenta expressed CD29, CD44, MRP1, ABCG2, P75NTR and Nestin, while CD34, CD133, HLA-DR and vWF were negative, and the fluorescence located in the cytoplasm.The mesenchymal stem cell-like cells derived from human umbilical cord and placenta express the surface antigens of the mesenchymal stem cell.

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备注/Memo

备注/Memo:
收稿日期:2011-10-20 △通信作者Email:xiaolingmr@163.com
更新日期/Last Update: 2011-12-30