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Title:: Research on the pO2 and Weak Fluorescence Monitoring and Imaging System in Photodynamic Therapy

Author(s):: HUANG Dong; LI Weitao; WANG Hongke; QIAN Zhiyu; Nanjing University of Aeronautics & Astronautics Biomedical Engineering, Nanjing 210016，China

Keywords:: Photodynamic therapy（PDT）; Partial pressure of oxygen; Weak fluorescence imaging; real-time monitor; Therapy evaluation

摘要:: 针对深部肿瘤光动力治疗（PDT）过程中关键参数的采集，我们开发了一套实时在体的氧分压和微弱荧光监测及成像系统。该系统利用普林斯顿高灵敏度CCD采集微弱荧光图像，利用海洋光学氧传感器NeoFox监测氧含量，采用LabVIEW开发上位机控制程序，将荧光图像、氧分压、荧光强度数据、结果的显示、分析与存储等功能结合在一起，实现了光动力治疗过程中实时多参数监测与成像。经光动力治疗反应荧光实验，验证了系统的有效性，为后续PDT疗效评估研究工作奠定了基础。

Abstract:: Since the key multiple parameters need to be monitored during the photodynamic therapy (PDT) in deep tumor, we developed a pO2 and weak fluorescence monitoring and imaging system. The Princeton Instrument scientific-grade CCD was used as the fluorescence imaging detector, NeoFox of Ocean Optics as O2 sensor and LabVIEW as programming platform. The function of the system included the fluorescence image measurement, the pO2 data acquisition, the fluorescence intensity detection, result display, data analyzing and recording simultaneously. The system realized the real-time multiple parameters measurement and analysis during PDT. The PDT chemical reaction experiments validate the sensitivity of system, which has a potential for assessment of PDT.