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Title:: Ultratrace Analysis of the Residual Monomer in Poly 2-Hydroxyethyl Methacry late Liquid Embolic Material by HPLC

Author(s):: ZHU Xiaojie; ZHU Xiaomin; MA Lixia; WANG Chuandong; XIA Yiran; MENG Jianwen; Shandong Academy of Pharmaceutical Sciences，Shandong Provincial Key Laboratory of Biomedical Polymers,Jinan 250101,China

Keywords:: HPLC; Liquid embolic material; 2-hydroxyethyl methacrylate; Residual monomer; Determination

摘要:: 应用高效液相色谱法测定了聚甲基丙烯酸-β-羟乙酯（PHEMA）液体栓塞材料中的单体甲基丙烯酸-β-羟乙酯（HEMA）残留量。采用色谱柱AichromBond-1 C18（4.6 mm×250 mm, 5 μm）进行分离，流动相为乙腈-水(30∶〖KG-*2〗70)；检测波长为220 nm；进样量为20 μL。单体在质量浓度为0.1～4.0 mg/L的范围，峰面积与单体浓度线性关系良好，相关系数0.9992，检测下限为0.01 mg/L。当试样加标浓度为0.5，2.0，4.0 mg/L时，加标回收率在99.5%~102.0%之间，RSD≤2%。

Abstract:: A method for determination of the residual monomer in poly 2-hydroxyethyl methacrylate(PHEMA) liquid embolic material by HPLC was established. AichromBond-1 C18 column（4.6mm×250mm, 5μm）was used as stationary phase and acetonitrile-H2O mixture (30:70) was used as mobile phase in the separation. Ultraviolet detector at 220 nm was adopted in the determination. The monomer showed a good calibration linearity with the range of 0.1～4.0 mg/L and the correlation coefficient was 0.9992. The detection limit is 0.01mg/L.When the sample concentration is 0.5，2.0，4.0 mg/L，the recoveries are ranged from 99.5% to 102.0%, and RSD≤2%.