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Title:: The Effect of Heparin- and Chondroitin Sulfate-based Nanoparticles Modified Surface on Biocompatibility

作者:: 曹建军1△; 刘涛2; 王国栋1; 汤潇涵2; 庞利群3; 潘长江2; 1. 山东省医学科学院附属医院设备科，山东济南 250031；2.淮阴工学院江苏省介入医疗器械研究重点实验室，江苏淮安 223003；3.淮安第一人民医院普外科，江苏淮安 223300

Author(s):: CAO Jianjun1; LIU Tao2; WANG Guodong1; TANG Xiaohan2; PANG Liqun3; PAN Changjiang2; 1.Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan 250031,China;2.Jiangsu Provincial Key Lab. for Interventional Medical Devices, Huaiyin Institute of Technology, Huai’an 223003,China;3.Department of General Surgery, Huai’an First People’s Hospital, Huai’an 223300

Keywords:: Dopamine; Chondroitin Sulfate; Heparin; Nanoparticles; Coagulation; Endothelialization

摘要:: 本研究合成了基于肝素和硫酸软骨素的两种纳米颗粒，用于316L不锈钢表面的生物功能改性。通过激光粒度分析仪、傅立叶变换红外光谱（FTIR）、原子力显微镜（AFM）、水接触角等对纳米颗粒的性质及颗粒固定前后表面的理化性质进行表征。通过体外血液相容性评价和内皮细胞相容性评价对两种纳米颗粒改性表面的生物相容性进行对比研究。结果表明，两种纳米颗粒均能有效降低材料表面血小板的粘附和聚集行为，但肝素纳米颗粒对内皮细胞的生长增殖表现出抑制作用，而硫酸软骨素纳米颗粒改性表面则具有促进内皮再生的潜能。

Abstract:: Heparin- and Chondroitin sulfate-based nanoparticles were synthesized in this study for biofunctional modification of 316L SS surface. The physical and chemical properties of NPs and NPs modified surface were characterized with laser particle size analysis, FTIR, AFM and water contact angle assay. In vitro blood compatibility and cellular compatibility were carried out to investigate the influence of two kinds of NPs on surface biocompatibility.〖JP2〗 According to the result, two kinds of NPs were found to effectively prevent platelet adhesion and aggregation. The heparin-based nanoparticle modified surface, however, may inhibit the proliferation activity of endothelial cells (ECs), while the chondroitin sulfate-based nanoparticles is found to stimulate ECs adhesion and proliferation.