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Title:: The biological delayed luminescence research of Scenedesmus as biological indicator

作者:: 张玉风1; 2; 杨美娜2; 庞靖祥2; 周宝宸1; 2; 韩金祥2△; 1.济南大学山东省医学科学院医学与生命科学学院，山东济南 250020；2.山东省医学科学院医药生物技术研究中心，山东济南 250062

Author(s):: ZHANG Yufeng1; 2; YANG Meina2; PANG Jingxiang2; ZHOU Baochen1; 2; HAN Jinxiang2△; 1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250020, China; 2. Shandong Academy of Medical Sciences, Shandong Medical Biotechnological Center, Jinan 250062

Keywords:: Scenedesmus; Biophoton; Delayed luminescence; Biological indicator; Conditions optimization; The Traditional Chinese Medicine

摘要:: 通过测量不同实验条件下栅藻的延迟发光动力学行为，确定最优实验条件，为栅藻作为生物指示剂奠定基础。用YPMS-2生物光子测量仪检测栅藻延迟发光，将测得的数据用Statistica 10.0软件进行拟合、处理，得到表征栅藻性质的可靠参数斜率k。栅藻的最佳使用浓度为2.5~3.5×107个/mL，栅藻使用的最佳生长阶段为40~60 d，加入不同中药煎煮液的栅藻斜率k值有较大差异，为研究中药药性提供了新的方向。

Abstract:: By measuring the delayed luminescence dynamics behavior of Scenedesmus in different experimental conditions, to determine the optimal experimental conditions, and lay a foundation for Scenedesmus as biological indicator. The delayed luminescence of Scenedesmus was detected with YPMS - 2 biophoton measuring instrument. The measured data were fitted and analyzed with statistica 10.0 software. Slope k, a reliable parameter that characterizes the nature of Scenedesmus is obtained. The optimal concentration range is (2.5~3.5)×107个/mL of using Scenedesmus. Growth of 40~60 d Scenedesmus is most suitable for use. The slope k value of Scenedesmus after adding different herbal decoction liquid has bigger differences. It provides a new direction for the study of Chinese medicinal properties.