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Title:: Type I collagen and isoproterenol-mediated branching formation of rat airway smooth muscle cells on 3D matrigel

Author(s):: QIAN Zhili ; DENG Linhong ; OUYANG Mingxing; Institute of Biomedical Engineering and Health Sciences,Changzhou University，Changzhou 213164,China

Keywords:: Type I collagen ; Airway smooth muscle cells ; Branching formation ; Asthma ; Biomechanics

摘要:: 细胞形态学发生与个体发育和组织生物工程相关。本研究初步探索了气道平滑肌细胞在三维matrigel胶上的形态发生，证实三维胶底培养条件和I型胶原是细胞群体自发生成分枝结构的必要微环境。临床缓解哮喘症状的气道舒张药物异丙肾上腺素有效抑制了该分枝形态发生，初步提示其需要生物力学因素的参与，同时该实验模型也可能具有检测临床气道舒张药物效果的功能。考虑到气道平滑肌具有收缩和舒张的特性，而在哮喘气道高反应现象中，平滑肌保持收缩状态，导致支气管通气受阻，因此，本研究也为进一步研究气道平滑肌细胞的力学特性提供了一个简便的模型。

Abstract:: The morphogenesis study is relevant to developmental processes and tissue bioengineering. In this work, we found rat airway smooth muscle cells self-assembled into branching structures on 3D matrigel, which process requires type I collagen (COL I) and the 3D microenvironment. Isoproterenol, a synthetic compound used for airway smooth muscle relaxation during asthma attack, can inhibit the branching formation, indicating a possible role of biomechanics in the branching, and a possible model to test clinical relaxation compounds. In considering that the airway smooth muscle has regular contraction and relaxation, whereas during airway hyper-responsiveness in asthma, the contraction forces sustain to prevent air flow through the bronchial tubes, hence this branching model may be used to further study the biomechanical features of airway smooth muscle cells.