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Title:: Design of tumor cell fluorescence detection system based on STM32 chip

Author(s):: JI Chunyang; XU Xiulin; School of Medical Instrument and Food Engineering，University of Shanghai for Science and Technology，Shanghai 200093，China

Keywords:: TM32; Micro-fluidic chip; Tumor cell; Fluorescence detection; Photoelectric conversion; Software design

摘要:: 为了实现肿瘤细胞早期筛查以达到早期防治，本研究开发了一种基于微流控芯片的肿瘤细胞荧光检测系统。光路采用反射式共焦距光路结构；硬件电路基于光电探测器模块、STM32F103C8T6、A/D转换芯片与串口芯片对激发荧光信号实时采集，并向上位机传输。同时设计了一套基于荧光检测的软件系统，实现检测动态可视化与报告生成及打印功能。采用FITC荧光素标记的乳腺癌细胞MDA-MB-453进行试验。结果表明，该系统在6 mL/h最佳进样速度下，对肿瘤细胞的平均检测率达到85.36%，为临床的应用提供可靠的诊断方法。

Abstract:: In order to achieve early screening of tumor cells for early prevention and control, We developed a tumor cell fluorescence detection system based on microfluidic chip. The optical path adopted a reflective confocal optical path structure; the hardware circuit was based on the photodetector module, STM32F103C8T6,A/D conversion chip and serial port chip, realized real-time acquisition of the excited fluorescent signal and transmission to the upper computer. At the same time, a software system based on fluorescence detection was designed to realize the dynamic visualization and the report generation and printing functions. The FITC-labeled breast cancer cell MDA-MB-453 was used to test the system. The results show that the average detection rate of tumor cells reach 85.36% at the optimal injection speed of 6 mL/h, which provides reliable diagnostic methods for clinical application.