文章摘要

文章信息/Info

Title:: Design and implementation of a circulating tumor cell sorting system based on air pressure drive

Author(s):: WANG Gubing; XU Xiulin; HU Xiufang; GUO Wanxin; School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology, Shanghai 200093,China

Keywords:: Circulating tumor cell sorting; STM32; Pressure control; Flow regulation; Injection system ; Proportion-integral-derivative

摘要:: 本研究开发了一种基于气压驱动的循环肿瘤细胞分选进样系统。采用上下位机控制结构，以STM32F103ZET6为气压控制核心，通过控制电路输出精度为0.001 V的控制电压信号，配合压力控制模块精确控制气压输出，并通过气体压力和液体流量采集模块,实时采集进样过程中的气体压力与液体流量参数，将液体流量信号作为比例-积分-微分控制器(proportion-integral-derivative，PID)的反馈量，实现系统的闭环流量调节，并将气体压力及液体流量通过上位机实时显示。对该进样系统用模拟外周血液进行不同流速下的进样实验，验证系统控制流量输出的可靠性。结果显示，其组内相关系数均大于0.85,证明该压力进样控制系统具有良好的可靠性，可为提高循环肿瘤细胞的分选效果提供技术保障。

Abstract:: We developed a pneumatically driven circulating tumor cell sorting system. It adopted the upper and lower machine control structure, with STM32F103ZET6 as the air pressure control core, the control circuit outputted the control voltage signal with the precision of 0.001 V, and the pressure control module precisely controlled the air pressure output, and collected the injection in real time through the gas pressure and liquid flow collection module. The gas pressure and liquid flow parameters in the process, the liquid flow signal was used as the feedback amount of the proportional-integral-derivative controller (PID) to realize the closed-loop flow regulation of the system, and the gas pressure and the liquid flow were displayed in real time through the upper computer. The injection system was used to simulate the peripheral blood at different flow rates to verify the reliability of the system control flow output. The results show that the correlation coefficient in the group is greater than 0.85, which proves that the pressure injection control system has good reliability and can provide technical guarantee for the improvement of the sorting effect of circulating tumor cells.