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Title:: Application of Raman spectroscopy in the identification of Wright-Giemsa stained tumor cells and normal cells

作者:: 李浩1; 于华杰2; 3; 李胜1; 2; 4; 韩露2; 沈阳洋2△; 1.山东省药物研究院，济南 250062；2.山东省肿瘤防治研究院，济南 250117；3.济南大学医学与生命科学学院，济南 250220；4.山东省医学科学院，济南 250062

Author(s):: LI Hao1; YU Huajie2; 3; LI Sheng1; 2; 4; HAN Lu2; SHEN Yangyang2; 1.Shandong Pharmaceutical Research Institute, Jinan 250062, China；2.Shangdong Cancer Hospital and Institute,Jinan 250117；3.School of Medicine and Life Sciences,University of Jinan,Jinan 250220；4.Shandong Academy of Medical Sciences, Jinan 250062

Keywords:: Raman spectroscopy; Raman spectral characteristic peak; WrightGiemsa staining; Tumor cells; Normal cell

摘要:: 为探究利用显微共聚焦拉曼光谱仪辨别经瑞氏-姬姆萨染色后的肿瘤细胞与正常细胞的可行性。应用拉曼光谱技术在532 nm波长激光下分别检测经瑞氏-姬姆萨染色技术染色的肿瘤细胞与正常细胞，对比6种肿瘤细胞、正常细胞和瑞氏-姬姆萨染料的拉曼光谱特征峰值，正常细胞和肿瘤细胞在208~215 cm-1、278~285 cm-1、336~341 cm-1峰位均有拉曼特征峰。发现6种肿瘤细胞的拉曼光谱峰值和瑞氏-姬姆萨染料的拉曼信号峰值均相同。经分析认为，检测到的均为各种染料的拉曼信号而非6种肿瘤细胞的拉曼信号。因此，无法用显微共聚焦拉曼光谱仪区分经瑞氏-姬姆萨染色后的肿瘤细胞与正常细胞。

Abstract:: To explore the feasibility of using micro confocal Raman spectrometer to discriminate tumor cells and normal cells stained by Wright-Giemsa dye,we used Raman spectroscopy to detect the tumor cells and normal cells with Wright-Giemsa stain under 532 nm wavelength laser, and compared the Raman spectral characteristic peaks of 6 kinds of tumor cells, normal cells and Wright-Giemsa dye.We found that the Raman peaks of 6 kinds tumor cells were the same as those of Wright-Giemsa dye. The results showed that the detected Raman signals were the Ramam signals of Wright-Giemsa dye, not those of 6 kinds tumor cells. Therefore, it is impossible to discriminate tumor cells and normal cells by using micro confocal Raman spectrometer.